Sortase-enzyme mediated coupling is a highly specific technology for site specific conjugation. It combines the specificity of transpeptidase Sortase A for short peptide sequences as recognition motifs (“tags”) with a high tolerance to a huge variety of payload and linker classes. The recognition sequences are incorporated into the light or heavy chain of an antibody so that the conjugation takes place exclusively at these positions (acceptor modifications) using a donor sequence modified linker.

Due to the highly selective nature of this process the Drug-to-Antibody-Ratio (DAR) is homogenously generated, thereby crafting ADCs with excellent pharmacokinetic properties (PK).

This process is established for GMP manufacturing.